Donald arrives at Krane's lair, only for Krane to siphon the power from the blaster, rendering it useless and quickly beating Donald with super strength and molecularkinesis. While the Lab Rats want to rescue them, Donald heads out alone with an extremely powerful thermal blaster. While Donald thinks it's Douglas, Krane reveals himself on-screen as the captor and demands that the Lab Rats arrive in 10 minutes. Back at the lab, a transmission comes in and shows Leo and Tasha captured. Douglas then shows Leo video footage of Krane implanting himself with bionics and asks him to call his brother to prove his honesty. While Leo is at Mission Creek High School, Douglas arrives and tries to appeal to Leo by reminding him that he knew about Douglas and Marcus before the rest of them. The Lab Rats instead believe this to be a trap, leading Donald to activate a new security system that only he can bypass. It is envisioned that viscous SC IFC paves the way for IFC to be really usable in practice with clogging-free, accurate, and sensitive performance.Donald shows a new motorcycle to the Lab Rats when they get a video message from Douglas that Krane has lost it and implanted himself with more bionic abilities, abilities even he doesn't know about. Viscous SC IFC also shows satisfactory ability to distinguish different types of cancer cells and different subtypes of human breast cancer cells. MC, and demonstrated that viscous non-conductive PEG solution achieved an improved sensitivity (7.92×) and signal-to-noise ratio (1.42×) in impedance measurement, with the accuracy maintained and free of clogging. Through modeling analysis and experiments, we confirmed the accuracy (error < 1.60% ± 4.71%) of SC w.r.t. By placing MC and SC in series in the same microfluidic chip, we established an evaluation platform to prove the hypothesis. Herein, we hypothesize that the viscosity of the non-conductive liquid is the key to the performance of SC, and propose to employ non-conductive viscous sheath flow in SC to unlock the tradeoff between sensitivity and throughput, while ensuring measurement accuracy. Current sheath constriction (SC) solutions lack systematic evaluation of the performance and proper guidelines for the sheath fluid. As a label-free and high-throughput single cell analysis platform, impedance flow cytometry (IFC) suffers from clogging caused by a narrow microchannel as mechanical constriction (MC).
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